Paraoxonase-1 and Glutathione Peroxidase Activity as a Screening Tool in Detecting Severity of Coronary Artery Disease: Case Control Feasibility Study

Dinushka Wickramasinghe; Hemantha Peiris; Lal Gotabhaya Chandrasena; Vajira Senaratne; Rasika Perera

doi:doi:10.11648/j.ajbls.20150306.14

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Paraoxonase-1 and Glutathione Peroxidase Activity as a Screening Tool in Detecting Severity of Coronary Artery Disease: Case Control Feasibility Study

Dinushka Wickramasinghe, Hemantha Peiris, Lal Gotabhaya Chandrasena, Vajira Senaratne, Rasika Perera

Published in American Journal of Biomedical and Life Sciences (Volume 3, Issue 6)

Received: 13 December 2015 Accepted: 23 December 2015 Published: 4 January 2016

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Abstract

Glutathione peroxidase and paraoxonase-1 is reported to be a useful marker for monitoring cardiovascular disease. Due to the paucity of information on the association of above markers and severity of coronary artery disease in South Asian patients, case control study was performed with 85 patients (58 males and 27 females) 40-60 years of age confirmed as having coronary artery disease on coronary angiography findings and 85 age and sex matched healthy volunteers as controls. Blood samples were analyzed for serum paraoxonase-1 and erythrocyte Glutathione peroxidase activity in both groups and the severity of coronary artery disease was assessed using coronary angiographic scoring system based on vessel, stenosis and extent score. Patients with coronary artery disease showed significantly low paraoxonase-1 and Glutathione peroxidase activity compared to control subjects. However, according to the best cutoff value determined by receiver operating characteristic analysis for serum paraoxonase-1 (38μg/mL) did not show a significantly high sensitivity, negative predictive value and negative likelihood ratio when compared to erythrocyte Glutathione peroxidase (84.5U/gHb) in predicting the severity of coronary artery disease assessed by three angiographic scores. Glutathione peroxidase appears to be an accurate marker in ruling out major coronary vessel disease and luminal narrowing by atheroma.

Published in	American Journal of Biomedical and Life Sciences (Volume 3, Issue 6)
DOI	10.11648/j.ajbls.20150306.14
Page(s)	120-126
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Glutathione Peroxidase, Paraoxonase, Vessel Score, Stenosis Score, Extent Score

References

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[17]	Zhou C, Cao J, Shang L, Tong C, Hu H, Wang H, et al. Reduced Paraoxonase 1 activity as a marker for severity of coronary artery disease. Disease Markers. 2013; 35: 97-103.
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APA Style

Dinushka Wickramasinghe, Hemantha Peiris, Lal Gotabhaya Chandrasena, Vajira Senaratne, Rasika Perera. (2016). Paraoxonase-1 and Glutathione Peroxidase Activity as a Screening Tool in Detecting Severity of Coronary Artery Disease: Case Control Feasibility Study. American Journal of Biomedical and Life Sciences, 3(6), 120-126. https://doi.org/10.11648/j.ajbls.20150306.14

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ACS Style

Dinushka Wickramasinghe; Hemantha Peiris; Lal Gotabhaya Chandrasena; Vajira Senaratne; Rasika Perera. Paraoxonase-1 and Glutathione Peroxidase Activity as a Screening Tool in Detecting Severity of Coronary Artery Disease: Case Control Feasibility Study. Am. J. Biomed. Life Sci. 2016, 3(6), 120-126. doi: 10.11648/j.ajbls.20150306.14

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AMA Style

Dinushka Wickramasinghe, Hemantha Peiris, Lal Gotabhaya Chandrasena, Vajira Senaratne, Rasika Perera. Paraoxonase-1 and Glutathione Peroxidase Activity as a Screening Tool in Detecting Severity of Coronary Artery Disease: Case Control Feasibility Study. Am J Biomed Life Sci. 2016;3(6):120-126. doi: 10.11648/j.ajbls.20150306.14

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@article{10.11648/j.ajbls.20150306.14,
  author = {Dinushka Wickramasinghe and Hemantha Peiris and Lal Gotabhaya Chandrasena and Vajira Senaratne and Rasika Perera},
  title = {Paraoxonase-1 and Glutathione Peroxidase Activity as a Screening Tool in Detecting Severity of Coronary Artery Disease: Case Control Feasibility Study},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {3},
  number = {6},
  pages = {120-126},
  doi = {10.11648/j.ajbls.20150306.14},
  url = {https://doi.org/10.11648/j.ajbls.20150306.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20150306.14},
  abstract = {Glutathione peroxidase and paraoxonase-1 is reported to be a useful marker for monitoring cardiovascular disease. Due to the paucity of information on the association of above markers and severity of coronary artery disease in South Asian patients, case control study was performed with 85 patients (58 males and 27 females) 40-60 years of age confirmed as having coronary artery disease on coronary angiography findings and 85 age and sex matched healthy volunteers as controls. Blood samples were analyzed for serum paraoxonase-1 and erythrocyte Glutathione peroxidase activity in both groups and the severity of coronary artery disease was assessed using coronary angiographic scoring system based on vessel, stenosis and extent score. Patients with coronary artery disease showed significantly low paraoxonase-1 and Glutathione peroxidase activity compared to control subjects. However, according to the best cutoff value determined by receiver operating characteristic analysis for serum paraoxonase-1 (38μg/mL) did not show a significantly high sensitivity, negative predictive value and negative likelihood ratio when compared to erythrocyte Glutathione peroxidase (84.5U/gHb) in predicting the severity of coronary artery disease assessed by three angiographic scores. Glutathione peroxidase appears to be an accurate marker in ruling out major coronary vessel disease and luminal narrowing by atheroma.},
 year = {2016}
}

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TY  - JOUR
T1  - Paraoxonase-1 and Glutathione Peroxidase Activity as a Screening Tool in Detecting Severity of Coronary Artery Disease: Case Control Feasibility Study
AU  - Dinushka Wickramasinghe
AU  - Hemantha Peiris
AU  - Lal Gotabhaya Chandrasena
AU  - Vajira Senaratne
AU  - Rasika Perera
Y1  - 2016/01/04
PY  - 2016
N1  - https://doi.org/10.11648/j.ajbls.20150306.14
DO  - 10.11648/j.ajbls.20150306.14
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 120
EP  - 126
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20150306.14
AB  - Glutathione peroxidase and paraoxonase-1 is reported to be a useful marker for monitoring cardiovascular disease. Due to the paucity of information on the association of above markers and severity of coronary artery disease in South Asian patients, case control study was performed with 85 patients (58 males and 27 females) 40-60 years of age confirmed as having coronary artery disease on coronary angiography findings and 85 age and sex matched healthy volunteers as controls. Blood samples were analyzed for serum paraoxonase-1 and erythrocyte Glutathione peroxidase activity in both groups and the severity of coronary artery disease was assessed using coronary angiographic scoring system based on vessel, stenosis and extent score. Patients with coronary artery disease showed significantly low paraoxonase-1 and Glutathione peroxidase activity compared to control subjects. However, according to the best cutoff value determined by receiver operating characteristic analysis for serum paraoxonase-1 (38μg/mL) did not show a significantly high sensitivity, negative predictive value and negative likelihood ratio when compared to erythrocyte Glutathione peroxidase (84.5U/gHb) in predicting the severity of coronary artery disease assessed by three angiographic scores. Glutathione peroxidase appears to be an accurate marker in ruling out major coronary vessel disease and luminal narrowing by atheroma.
VL  - 3
IS  - 6
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Author Information

Dinushka Wickramasinghe

Department of Biochemistry, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka
Hemantha Peiris

Department of Biochemistry, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka
Lal Gotabhaya Chandrasena

Nawaloka Hospitals, Colombo, Sri Lanka
Vajira Senaratne

Cardiology Unit, National Hospital Sri Lanka, Colombo 07, Sri Lanka
Rasika Perera

Department of Biochemistry, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka

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APA Style

Dinushka Wickramasinghe, Hemantha Peiris, Lal Gotabhaya Chandrasena, Vajira Senaratne, Rasika Perera. (2016). Paraoxonase-1 and Glutathione Peroxidase Activity as a Screening Tool in Detecting Severity of Coronary Artery Disease: Case Control Feasibility Study. American Journal of Biomedical and Life Sciences, 3(6), 120-126. https://doi.org/10.11648/j.ajbls.20150306.14

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ACS Style

Dinushka Wickramasinghe; Hemantha Peiris; Lal Gotabhaya Chandrasena; Vajira Senaratne; Rasika Perera. Paraoxonase-1 and Glutathione Peroxidase Activity as a Screening Tool in Detecting Severity of Coronary Artery Disease: Case Control Feasibility Study. Am. J. Biomed. Life Sci. 2016, 3(6), 120-126. doi: 10.11648/j.ajbls.20150306.14

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AMA Style

Dinushka Wickramasinghe, Hemantha Peiris, Lal Gotabhaya Chandrasena, Vajira Senaratne, Rasika Perera. Paraoxonase-1 and Glutathione Peroxidase Activity as a Screening Tool in Detecting Severity of Coronary Artery Disease: Case Control Feasibility Study. Am J Biomed Life Sci. 2016;3(6):120-126. doi: 10.11648/j.ajbls.20150306.14

Copy | Download

@article{10.11648/j.ajbls.20150306.14,
  author = {Dinushka Wickramasinghe and Hemantha Peiris and Lal Gotabhaya Chandrasena and Vajira Senaratne and Rasika Perera},
  title = {Paraoxonase-1 and Glutathione Peroxidase Activity as a Screening Tool in Detecting Severity of Coronary Artery Disease: Case Control Feasibility Study},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {3},
  number = {6},
  pages = {120-126},
  doi = {10.11648/j.ajbls.20150306.14},
  url = {https://doi.org/10.11648/j.ajbls.20150306.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20150306.14},
  abstract = {Glutathione peroxidase and paraoxonase-1 is reported to be a useful marker for monitoring cardiovascular disease. Due to the paucity of information on the association of above markers and severity of coronary artery disease in South Asian patients, case control study was performed with 85 patients (58 males and 27 females) 40-60 years of age confirmed as having coronary artery disease on coronary angiography findings and 85 age and sex matched healthy volunteers as controls. Blood samples were analyzed for serum paraoxonase-1 and erythrocyte Glutathione peroxidase activity in both groups and the severity of coronary artery disease was assessed using coronary angiographic scoring system based on vessel, stenosis and extent score. Patients with coronary artery disease showed significantly low paraoxonase-1 and Glutathione peroxidase activity compared to control subjects. However, according to the best cutoff value determined by receiver operating characteristic analysis for serum paraoxonase-1 (38μg/mL) did not show a significantly high sensitivity, negative predictive value and negative likelihood ratio when compared to erythrocyte Glutathione peroxidase (84.5U/gHb) in predicting the severity of coronary artery disease assessed by three angiographic scores. Glutathione peroxidase appears to be an accurate marker in ruling out major coronary vessel disease and luminal narrowing by atheroma.},
 year = {2016}
}

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TY  - JOUR
T1  - Paraoxonase-1 and Glutathione Peroxidase Activity as a Screening Tool in Detecting Severity of Coronary Artery Disease: Case Control Feasibility Study
AU  - Dinushka Wickramasinghe
AU  - Hemantha Peiris
AU  - Lal Gotabhaya Chandrasena
AU  - Vajira Senaratne
AU  - Rasika Perera
Y1  - 2016/01/04
PY  - 2016
N1  - https://doi.org/10.11648/j.ajbls.20150306.14
DO  - 10.11648/j.ajbls.20150306.14
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 120
EP  - 126
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20150306.14
AB  - Glutathione peroxidase and paraoxonase-1 is reported to be a useful marker for monitoring cardiovascular disease. Due to the paucity of information on the association of above markers and severity of coronary artery disease in South Asian patients, case control study was performed with 85 patients (58 males and 27 females) 40-60 years of age confirmed as having coronary artery disease on coronary angiography findings and 85 age and sex matched healthy volunteers as controls. Blood samples were analyzed for serum paraoxonase-1 and erythrocyte Glutathione peroxidase activity in both groups and the severity of coronary artery disease was assessed using coronary angiographic scoring system based on vessel, stenosis and extent score. Patients with coronary artery disease showed significantly low paraoxonase-1 and Glutathione peroxidase activity compared to control subjects. However, according to the best cutoff value determined by receiver operating characteristic analysis for serum paraoxonase-1 (38μg/mL) did not show a significantly high sensitivity, negative predictive value and negative likelihood ratio when compared to erythrocyte Glutathione peroxidase (84.5U/gHb) in predicting the severity of coronary artery disease assessed by three angiographic scores. Glutathione peroxidase appears to be an accurate marker in ruling out major coronary vessel disease and luminal narrowing by atheroma.
VL  - 3
IS  - 6
ER  -

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